/
/
عزل CD 90+ الخلايا الليفية / Myofibroblasts من المجمدة الإنسان الجهاز الهضمي العينات
A subscription to JoVE is required to view this content. Sign in or start your free trial.
9,314 Views
•
06:02 min
•
January 31, 2016
DOI:
Summary
Automatically generated
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
Read Article
Cite this Article
Johnson, P., Beswick, E. J., Chao, C., Powell, D. W., Hellmich, M. R., Pinchuk, I. V. Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens. J. Vis. Exp. (107), e53691, doi:10.3791/53691 (2016).
Copy