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Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
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Chemistry
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JoVE Journal Chemistry
Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

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8,124 Views

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14:32 min

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February 27, 2016

DOI:

10.3791/53563-v

DOI:
10.3791/53563-v

•

14:32 min
February 27, 2016

•

2 Views
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1Laboratory for Molecular Cancer Biology, Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center,Feinberg School of Medicine, Northwestern University, 2Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center,Feinberg School of Medicine, Northwestern University

Summary

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This protocol describes the required steps to execute in vitro and in vivo deacetylation assays in order to establish the role of proteins as specific deacetylation substrates for sirtuins and further study the role of reversible - lysine acetylation as a post-translational modification.

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Cite this Article

Song, H. Y., Park, S., Kang, H., Vassilopoulos, A. Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates. J. Vis. Exp. (108), e53563, doi:10.3791/53563 (2016).

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