We describe a Xenopus oocyte and animal cap system for the expression cloning of genes capable of inducing a response in competent ectoderm, and discuss techniques for the subsequent analysis of such genes. This system is useful in the functional identification of a wide range of gene products.
Plautz, C. Z., Williams, H. C., Grainger, R. M. Functional Cloning Using a Xenopus Oocyte Expression System. J. Vis. Exp. (107), e53518, doi:10.3791/53518 (2016).