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Immunology and Infection
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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

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7,784 Views

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09:13 min

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January 13, 2016

DOI:

10.3791/53460-v

DOI:
10.3791/53460-v

•

09:13 min
January 13, 2016

•

1 Views
, , ,
1Department of Biological Sciences,Carnegie Mellon University, 2Infectious Diseases,Harbor-UCLA Medical Center

Summary

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We developed a protocol that is fast, sensitive and reproducible for pathogen gene expression profiling during an infection.

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Gene Expression ProfilingDigital Bar-codingPathogen Gene ExpressionInfectious DiseaseC. AlbicansRNA ExtractionPhenol-chloroform Extraction

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Xu, W., Solis, N. V., Filler, S. G., Mitchell, A. P. Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform. J. Vis. Exp. (107), e53460, doi:10.3791/53460 (2016).

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