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Journal
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Chemistry
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光驱动脱羧酶
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Light-driven Enzymatic Decarboxylation
Please note that all translations are automatically generated.
Click here for the English version.
光驱动脱羧酶
DOI:
10.3791/53439-v
•
09:58 min
•
May 22, 2016
•
Katharina Köninger
,
Marius Grote
,
Ioannis Zachos
,
Frank Hollmann
,
Robert Kourist
1
Faculty of Biology and Biotechnology
,
Ruhr Universität Bochum
,
2
Biology and Biotechnology
,
Delft University of Technology
Chapters
00:05
Title
01:00
Production of Recombinant Decarboxylase in
Escherichia coli
03:42
Purification of OleT
JE
05:22
Biotransformation of Fatty Acids Using OleT
06:50
Analysis of Biotransformation Products
08:26
Results: Analysis of the Light-catalyzed Biotransformations by Gas Chromatography
09:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述用于光催化生成过氧化氢的一个协议 - 对于氧化转化的辅因子。
Tags
Enzymatic Decarboxylation
Light-driven Biocatalysis
OleT Enzyme
Fatty Acid Decarboxylation
Terminal Alkyne Synthesis
Hydrogen Peroxide Generation
E. Coli Expression
Protein Purification
Cell Lysis
Cell-free Extract
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