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Murin fibroblastes dermiques Isolation par FACS
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Murine Dermal Fibroblast Isolation by FACS

Murin fibroblastes dermiques Isolation par FACS

20,108 Views

06:04 min

January 07, 2016

DOI:

06:04 min
January 07, 2016

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Summary

Automatically generated

Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.

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