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リアルタイム細胞外フラックス分析を用いた偏光M1、M2の骨髄由来マクロファージの代謝特性評価
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
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リアルタイム細胞外フラックス分析を用いた偏光M1、M2の骨髄由来マクロファージの代謝特性評価

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34,479 Views

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07:45 min

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November 28, 2015

DOI:

10.3791/53424-v

DOI:
10.3791/53424-v

•

07:45 min
November 28, 2015

•

28 Views
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1Department of Medical Biochemistry, Experimental Vascular Biology,Academic Medical Center, Amsterdam

Summary

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Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

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Macrophage PolarizationM1 MacrophagesM2 MacrophagesMetabolic ReprogrammingGlycolysisOxidative PhosphorylationExtracellular Flux AnalysisOxygen Consumption RateExtracellular Acidification RateMitochondrial ActivitySpare Respiratory Capacity

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Cite this Article

Van den Bossche, J., Baardman, J., de Winther, M. P. Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis. J. Vis. Exp. (105), e53424, doi:10.3791/53424 (2015).

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