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Эффективное протокол Изоляция для B и Т-лимфоциты из небных миндалин человека
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Immunology and Infection
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JoVE Journal Immunology and Infection
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
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Эффективное протокол Изоляция для B и Т-лимфоциты из небных миндалин человека

русский

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15,538 Views

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08:09 min

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November 16, 2015

DOI:

10.3791/53374-v

DOI:
10.3791/53374-v

•

08:09 min
November 16, 2015

•

5 Views
, , , , ,
1Department of Medical Biochemistry and Microbiology,Uppsala Biomedical Center, Uppsala University, 2Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery,Akademiska sjukhuset

Summary

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Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.

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Cite this Article

Assadian, F., Sandström, K., Laurell, G., Svensson, C., Akusjärvi, G., Punga, T. Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils. J. Vis. Exp. (105), e53374, doi:10.3791/53374 (2015).

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