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Bacteriana Folha Infiltração Ensaio para Belas Caracterização das respostas de defesa da planta usando o Arabidopsis thaliana-Pseudomonas syringae Patossistema
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacteriana Folha Infiltração Ensaio para Belas Caracterização das respostas de defesa da planta usando o Arabidopsis thaliana-Pseudomonas syringae Patossistema

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21,329 Views

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11:50 min

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October 01, 2015

DOI:

10.3791/53364-v

DOI:
10.3791/53364-v

•

11:50 min
October 01, 2015

•

8 Views
, , , , ,
1Department of Biology,University of Alabama at Birmingham

Summary

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Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.

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Liu, X., Sun, Y., Kørner, C. J., Du, X., Vollmer, M. E., Pajerowska-Mukhtar, K. M. Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem. J. Vis. Exp. (104), e53364, doi:10.3791/53364 (2015).

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