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自動化されたモジュラーハイスループットエキソポリサッカライドスクリーニングプラットフォームは、高感度炭水化物フィンガープリント分析と相まって
JoVE Journal
Biology
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JoVE Journal Biology
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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自動化されたモジュラーハイスループットエキソポリサッカライドスクリーニングプラットフォームは、高感度炭水化物フィンガープリント分析と相まって

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11,240 Views

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12:02 min

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April 11, 2016

DOI:

10.3791/53249-v

DOI:
10.3791/53249-v

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12:02 min
April 11, 2016

•

7 Views
, ,
1Chair of Chemistry of Biogenic Resources,Technische Universität München

Summary

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We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.

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ExopolysaccharideAutomated ScreeningCarbohydrate FingerprintHigh ThroughputModular PlatformMicrobial DiversityNovel Polysaccharide VariantsAutomated Robotic ProgramCentrifugationFiltrationPrecipitation

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Cite this Article

Rühmann, B., Schmid, J., Sieber, V. Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis. J. Vis. Exp. (110), e53249, doi:10.3791/53249 (2016).

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