Journal
/
Neuroscience
/
فرز خلية العصبية الجذعية وخلايا السلف من الفأر Subventricular المنطقة الكبار وحية على التصوير من حيوية دورة الخلية الخاصة
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
Please note that all translations are automatically generated. Click here for the English version.

فرز خلية العصبية الجذعية وخلايا السلف من الفأر Subventricular المنطقة الكبار وحية على التصوير من حيوية دورة الخلية الخاصة

العربية

Automatically Generated

13,867 Views

•

09:27 min

•

September 14, 2015

DOI:

10.3791/53247-v

DOI:
10.3791/53247-v

•

09:27 min
September 14, 2015

•

1 Views
, , , , , ,
1CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, 2INSERM, UMR 967, 3Université Paris Diderot, Sorbonne Paris Cité, UMR 967, 4Université Paris Sud, UMR 967, 5CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

Summary

Automatically generated

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.

Explore More Videos

Neural Stem CellsSubventricular ZoneCell SortingFluorescence-activated Cell Sorting (FACS)Cell Cycle DynamicsTransit Amplifying CellsNeuroblastsFUCCILive-imagingAgingNeurogenesis

Read Article

Cite this Article

Daynac, M., Morizur, L., Kortulewski, T., Gauthier, L. R., Ruat, M., Mouthon, M., Boussin, F. D. Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics. J. Vis. Exp. (103), e53247, doi:10.3791/53247 (2015).

Download .ris file

Copy

Share Video

.

Copy