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August 03, 2015
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This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.
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Jung, J., Kim, B. S. A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media. J. Vis. Exp. (102), e53204, doi:10.3791/53204 (2015).
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