/
/
TCR-pMHC Bağlama Ölçüm<em> In Situ</em> Bir FRET tabanlı Mikroskopi Assay kullanılarak
This content is Free Access.
12,034 Views
•
19:05 min
•
October 30, 2015
DOI:
Summary
Automatically generated
This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.
Read Article
Cite this Article
Axmann, M., Schütz, G. J., Huppa, J. B. Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay. J. Vis. Exp. (104), e53157, doi:10.3791/53157 (2015).
Copy