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Enriquecimiento de la Matriz Extracelular Las proteínas de los tejidos y la digestión en péptidos para el análisis de espectrometría de masas
JoVE Journal
Biology
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JoVE Journal Biology
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
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Enriquecimiento de la Matriz Extracelular Las proteínas de los tejidos y la digestión en péptidos para el análisis de espectrometría de masas

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26,071 Views

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07:28 min

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July 23, 2015

DOI:

10.3791/53057-v

DOI:
10.3791/53057-v

•

07:28 min
July 23, 2015

•

31 Views
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1Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Proteomics Platform,Broad Institute

Summary

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This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

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Extracellular MatrixECMDigestionPeptidesMass SpectrometryDecellularizationEnrichmentCross-linked ProteinsGlycosylationBiomarkersDiagnosticTherapeutic Targets

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Cite this Article

Naba, A., Clauser, K. R., Hynes, R. O. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis. J. Vis. Exp. (101), e53057, doi:10.3791/53057 (2015).

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