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Phenomics de phages: approches physiologiques à caractériser de nouvelles protéines virales
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June 11, 2015
DOI:
1Department of Biology,San Diego State University, 2Computational Science Research Center,San Diego State University, 3Bioinformatics and Medical Informatics Research Center,San Diego State University, 4Department of Mathematics and Statistics,San Diego State University, 5Department of Computer Science,San Diego State University, 6Mathematics and Computer Science Division,Argonne National Laboratory, 7SPARC Committee,Broad Institute
Summary
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Here, we present phenomic approaches for the functional characterization of putative phage genes. Techniques include a developed assay capable of monitoring host anabolic metabolism, the Multi-phenotype Assay Plates (MAPs), in addition to the established method of metabolomics, capable of measuring effects to catabolic metabolism.
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Sanchez, S. E., Cuevas, D. A., Rostron, J. E., Liang, T. Y., Pivaroff, C. G., Haynes, M. R., Nulton, J., Felts, B., Bailey, B. A., Salamon, P., Edwards, R. A., Burgin, A. B., Segall, A. M., Rohwer, F. Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins. J. Vis. Exp. (100), e52854, doi:10.3791/52854 (2015).
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