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Aplicação do DNA-metil específico Stain Verde no Labeling fluorescentes de Embriões
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May 02, 2015
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Summary
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A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.
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Prieto, D., Aparicio, G., Machado, M., Zolessi, F. R. Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos. J. Vis. Exp. (99), e52769, doi:10.3791/52769 (2015).
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