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Journal
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Immunology and Infection
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荧光标记基因的基因枪转化成机会性真菌病原<em>新型隐球菌</em
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen
Cryptococcus neoformans
Please note that all translations are automatically generated.
Click here for the English version.
荧光标记基因的基因枪转化成机会性真菌病原<em>新型隐球菌</em
DOI:
10.3791/52666-v
•
07:32 min
•
March 19, 2015
•
Tonya Taylor
,
Indrani Bose
,
Taylor Luckie
,
Kerry Smith
1
Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC)
,
Clemson University
,
2
Department of Biology
,
Western Carolina University
Chapters
00:05
Title
01:07
DNA Preparation
02:41
Gene Gun Operation
04:59
Plating Transformed Cells
05:52
Results: Successful Homologous Recombination
07:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
生物射弹转化是用于产生的DNA的稳定整合入条件致病菌
新生隐球菌
通过同源重组的基因组中的方法。我们将展示一个结构,它具有融合到荧光标记mCherry到
新生隐球菌
基因编码醋酸激酶的基因枪转化。
Tags
Biolistic Transformation
Fluorescent Tagged Gene
Cryptococcus Neoformans
Opportunistic Fungal Pathogen
Acetate Production
Xylulose-5-phosphate/fructose-6-phosphate Phosphoketolase
Acetate Kinase
MCherry
Homologous Recombination
Fungal Meningitis
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