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协议获得合子和体细胞胚胎研究早期胚胎发育的调控中的示范豆科<em>苜蓿</em
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JoVE Journal Developmental Biology
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
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协议获得合子和体细胞胚胎研究早期胚胎发育的调控中的示范豆科<em>苜蓿</em

English

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13,721 Views

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07:32 min

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June 09, 2015

DOI:

10.3791/52635-v

DOI:
10.3791/52635-v

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07:32 min
June 09, 2015

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2 Views
, , , , ,
1School of Environmental and Life Sciences,University of Newcastle, Callaghan, New South Wales, Australia

Summary

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The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.

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Kurdyukov, S., Song, Y., Tiew, T. W., Wang, X., Nolan, K. E., Rose, R. J. Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula. J. Vis. Exp. (100), e52635, doi:10.3791/52635 (2015).

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