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JoVE Journal Developmental Biology
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
 

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Article DOI: 10.3791/52635-v • 07:32 min • June 9th, 2015
June 9th, 2015 •
1, 1, 1, 1, 1, 1
1School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia

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The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.

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Keywords: Zygotic Embryos Somatic Embryos Medicago Truncatula Early Embryo Development Embryogenesis Pod Morphology Leaf Explants Auxin Cytokinin Abscisic Acid Gibberellic Acid
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