Journal
/
Developmental Biology
/
小分子変調分化とその後のグルコース飢餓を用いたヒト人工多能性幹細胞からの高度に精製された心筋細胞の導出
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
Please note that all translations are automatically generated. Click here for the English version.

小分子変調分化とその後のグルコース飢餓を用いたヒト人工多能性幹細胞からの高度に精製された心筋細胞の導出

日本語

Automatically Generated

18,249 Views

•

11:53 min

•

March 18, 2015

DOI:

10.3791/52628-v

DOI:
10.3791/52628-v

•

11:53 min
March 18, 2015

•

4 Views
, , , , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Institute of Stem Cell Biology and Regenerative Medicine, Cardiovascular Medicine Division, Department of Medicine, Child Health Research Institute,Stanford University School of Medicine

Summary

Automatically generated

Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.

Explore More Videos

Human Induced Pluripotent Stem CellsCardiomyocyte DifferentiationSmall Molecule-based ProtocolWnt/β-Catenin SignalingGlucose StarvationPurified CardiomyocytesIn Vitro Disease ModelingDrug Screening

Read Article

Cite this Article

Sharma, A., Li, G., Rajarajan, K., Hamaguchi, R., Burridge, P. W., Wu, S. M. Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation. J. Vis. Exp. (97), e52628, doi:10.3791/52628 (2015).

Download .ris file

Copy

Share Video

.

Copy