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干血斑点. 免疫和分子技术中使用的制备和加工

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques

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干血斑点. 免疫和分子技术中使用的制备和加工

38,151 Views

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11:28 min

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March 13, 2015

DOI:

10.3791/52619-v

DOI:

10.3791/52619-v

•

11:28 min

March 13, 2015

•

46 Views

Nico Grüner¹, Oumaima Stambouli¹, R. Stefan Ross¹

¹Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital,University of Duisburg-Essen

Summary

在大多数诊断应用中, 对干血点 (DBS) 进行最终分析的准备和处理仍不规范。为了克服这一缺点, 提出了一个全面的 step-by 步协议, 并随后对其检测病毒感染标志的有效性进行了评估。

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