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Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques
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生物学
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JoVE Journal 生物学
Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques

38,343 Views

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11:28 min

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March 13, 2015

DOI:

10.3791/52619-v

DOI:
10.3791/52619-v

•

11:28 min
March 13, 2015

•

46 Views
, ,
1Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital,University of Duisburg-Essen

概要

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The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.

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Grüner, N., Stambouli, O., Ross, R. S. Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques. J. Vis. Exp. (97), e52619, doi:10.3791/52619 (2015).

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