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تقنية وقت التفاضلية تلطيخ جانب كامل الثنائي جيل إزالة التعصيب لدراسة Ionocytes في السمك
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
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تقنية وقت التفاضلية تلطيخ جانب كامل الثنائي جيل إزالة التعصيب لدراسة Ionocytes في السمك

العربية

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8,245 Views

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11:24 min

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March 19, 2015

DOI:

10.3791/52548-v

DOI:
10.3791/52548-v

•

11:24 min
March 19, 2015

•

1 Views
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1Department of Biology,University of Ottawa

Summary

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This manuscript describes a protocol to track the re-distribution of branchial ionocytes and their innervation using a time differential staining technique coupled with full bilateral gill denervation.

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Gill IonocytesTime Differential StainingFull Bilateral Gill DenervationMitochondrion-specific DyeNa+/K+ ATPaseImmunohistochemistryConfocal ImagingFish GillIonic Regulation

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Tzaneva, V., Perry, S. F. A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish. J. Vis. Exp. (97), e52548, doi:10.3791/52548 (2015).

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