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トランスフェクション、選択、およびAAVS1セーフハーバーへのGFP遺伝子とTALENを標的とする人間の人工多能性幹細胞のコロニーピッキング
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トランスフェクション、選択、およびAAVS1セーフハーバーへのGFP遺伝子とTALENを標的とする人間の人工多能性幹細胞のコロニーピッキング
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07:28 min
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February 01, 2015
DOI:
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TransfectionSelectionColony-pickingHuman Induced Pluripotent Stem CellsTALENGFPAAVS1 Safe HarborTargeted Transgene AdditionGene EditingGene SilencingInsertional MutagenesisViral VectorGene-trap-like DonorSingle Cell DissociationNuclear Delivery EfficiencyPuromycin SelectionPPP1R12CSplicing AcceptorPACTargeted ClonesGenetic ScreeningFunctional ScreeningGFP Reporter IPSC Lines
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Cerbini, T., Luo, Y., Rao, M. S., Zou, J. Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor. J. Vis. Exp. (96), e52504, doi:10.3791/52504 (2015).
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