Journal
/
Immunology and Infection
/
グラスサポート平面脂質二重層上のナチュラルキラー細胞免疫シナプスの超解像イメージング
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
Please note that all translations are automatically generated. Click here for the English version.

グラスサポート平面脂質二重層上のナチュラルキラー細胞免疫シナプスの超解像イメージング

日本語

Automatically Generated

12,151 Views

•

09:56 min

•

February 11, 2015

DOI:

10.3791/52502-v

DOI:
10.3791/52502-v

•

09:56 min
February 11, 2015

•

5 Views
, , , ,
1Center for Human Immunobiology,Texas Children’s Hospital, 2Department of Pediatrics,Baylor College of Medicine, 3Department of Pathology and Immunology,Baylor College of Medicine

Summary

Automatically generated

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

Explore More Videos

Super-resolution ImagingNatural Killer CellImmunological SynapseGlass-supported Planar Lipid BilayerSTED MicroscopyPerforinFilamentous ActinNK Cell Activation Receptor CD16

Read Article

Cite this Article

Zheng, P., Bertolet, G., Chen, Y., Huang, S., Liu, D. Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer. J. Vis. Exp. (96), e52502, doi:10.3791/52502 (2015).

Download .ris file

Copy

Share Video

.

Copy