A PCR-based protocol was adapted to detect Cronobacter spp., Salmonella enterica, and Listeria monocytogenes from body surfaces and alimentary canals of individual wild-caught flies. The goal of this protocol is to detect and isolate bacterial pathogens from individual insects collected as part of an environmental sampling program during foodborne outbreak investigations.
Pava-Ripoll, M., Pearson, R. E., Miller, A. K., Ziobro, G. C. Detection of Foodborne Bacterial Pathogens from Individual Filth Flies. J. Vis. Exp. (96), e52372, doi:10.3791/52372 (2015).