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A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
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JoVE Journal Environment
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

English

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10,272 Views

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13:16 min

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December 24, 2014

DOI:

10.3791/52334-v

DOI:
10.3791/52334-v

•

13:16 min
December 24, 2014

•

3 Views
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1Department of Environmental Microbiology,Helmholtz Centre for Environmental Research – UFZ, 2Department of River Ecology,Helmholtz Centre for Environmental Research – UFZ

Summary

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A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.

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BioavailabilityContaminant TransportUnsaturated SoilMyceliaBioreporterPAHFluoreneBurkholderiaPythium UltimumConfocal Laser Scanning Microscopy

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Cite this Article

Schamfuß, S., Neu, T. R., Harms, H., Wick, L. Y. A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems. J. Vis. Exp. (94), e52334, doi:10.3791/52334 (2014).

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