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January 09, 2015
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This paper describes the cryo-electron microscopy methodology, which is used to obtain high quality microscopic images of macromolecules in their near-native state. The method yields images suitable for further computerized processing using the single particle approach, devised to generate the 3D reconstruction of a macromolecule.
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Cryo-electron MicroscopyCryoEMSample PreparationMacromolecular 3D ReconstructionTransmission Electron MicroscopyTEMSingle-particle Image Processing3D Structural DeterminationPurificationVitrificationImage Processing SoftwareStructural DeterminationMacromolecular MachinesPseudo-atomic StructuresAtomic Resolution
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Cabra, V., Samsó, M. Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction. J. Vis. Exp. (95), e52311, doi:10.3791/52311 (2015).
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