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Fotoçevrim sonra Floresans Bozunma kullanarak Oturma balığı Embriyolar protein stabilitesi ölçümü (FDAP)
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Developmental Biology
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JoVE Journal Developmental Biology
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
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Fotoçevrim sonra Floresans Bozunma kullanarak Oturma balığı Embriyolar protein stabilitesi ölçümü (FDAP)

Türkçe

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11,083 Views

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09:45 min

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January 28, 2015

DOI:

10.3791/52266-v

DOI:
10.3791/52266-v

•

09:45 min
January 28, 2015

•

4 Views
, , ,
1Department of Molecular and Cellular Biology,Harvard University, 2Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Summary

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Hücre ve dokularda protein seviyeleri, çoğu zaman sıkı bir şekilde protein üretimi ve temizlenmesi dengesi ile düzenlenir. Fotoçevrim (FDAP) sonra floresans Bozunma kullanan proteinlerin temizlenme kinetiği deneysel in vivo olarak ölçülebilir.

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Cite this Article

Rogers, K. W., Bläßle, A., Schier, A. F., Müller, P. Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP). J. Vis. Exp. (95), e52266, doi:10.3791/52266 (2015).

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