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Het isoleren van Intestinale stamcellen van volwassen<em> Drosophila</em> Midguts door FACS om Stem Cell gedrag te bestuderen tijdens veroudering
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Biology
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JoVE Journal Biology
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
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Het isoleren van Intestinale stamcellen van volwassen<em> Drosophila</em> Midguts door FACS om Stem Cell gedrag te bestuderen tijdens veroudering

Nederlands

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19,747 Views

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10:57 min

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December 16, 2014

DOI:

10.3791/52223-v

DOI:
10.3791/52223-v

•

10:57 min
December 16, 2014

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1 Views
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1Institut für Biochemie und Molekulare Biologie,Universität Ulm, 2Institut für Immunologie,Universitätsklinikum Ulm

Summary

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Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

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Cite this Article

Tauc, H. M., Tasdogan, A., Pandur, P. Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging. J. Vis. Exp. (94), e52223, doi:10.3791/52223 (2014).

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