Journal
/
Immunology and Infection
/
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

English

Automatically Generated

11,684 Views

•

10:02 min

•

December 12, 2014

DOI:

10.3791/52100-v

DOI:
10.3791/52100-v

•

10:02 min
December 12, 2014

•

11 Views
, , , ,
1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968,Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation,Fordham University

Summary

Automatically generated

This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.

Explore More Videos

Photoreceptor Outer SegmentsRetinal Pigment Epithelial CellsPhagocytosis AssayPorcineBovineSucrose GradientPhotopigmentFITC LabelingFluorescence QuantificationOxidative StressAging

Read Article

Cite this Article

Parinot, C., Rieu, Q., Chatagnon, J., Finnemann, S. C., Nandrot, E. F. Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells. J. Vis. Exp. (94), e52100, doi:10.3791/52100 (2014).

Download .ris file

Copy

Share Video

.

Copy