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تحديد تركيز الفيروسي من خلال البلاك فحوصات: نظم تراكب عن طريق التقليدية والرواية
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

تحديد تركيز الفيروسي من خلال البلاك فحوصات: نظم تراكب عن طريق التقليدية والرواية

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09:28 min

November 04, 2014

DOI:

09:28 min
November 04, 2014

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Summary

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Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

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