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幼虫RNA干扰在赤拟谷盗，<em>赤拟谷盗</em

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

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幼虫RNA干扰在赤拟谷盗，<em>赤拟谷盗</em

DOI:

10.3791/52059-v

•

11:00 min

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October 13, 2014

•

David M. Linz*¹, Courtney M. Clark-Hachtel*¹, Ferran Borràs-Castells*¹, Yoshinori Tomoyasu

¹Department of Biology,Miami University

Chapters

00:05Title
02:12Isolation and Selection of T. castaneum Larvae
03:35Preparation of Injection Needles
04:39Frontloading dsRNA Solution into the Needle
06:06dsRNA Injection
08:05Results: Microinjections of T. castaneum
09:52Conclusion

Summary

Automatic Translation

RNA干扰（RNAi）技术为基础的基因敲除技术是在赤研究的核心。这里，我们提供了在赤拟谷盗我们的幼虫RNAi技术的概览。幼虫RNAi是一种简单，但功能强大的技术，可以快速访问功能丧失的表型，使研究人员能够研究基因的功能在不同的上下文中。

Tags

Tribolium Castaneum Red Flour Beetle RNA Interference RNAi Larval RNAi Gene Knockdown Transgenesis Genetic System Developmental Studies

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