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August 21, 2014
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The ability to isolate heart valve endothelial cells (VECs) is critical for understanding mechanisms of valve development, maintenance, and disease. Here we describe the isolation of VECs from embryonic and adult Tie2-GFP mice using FACS that will allow for studies determining the contribution of VECs in developmental and disease processes.
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Miller, L. J., Lincoln, J. Isolation of Murine Valve Endothelial Cells. J. Vis. Exp. (90), e51860, doi:10.3791/51860 (2014).
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