A novel method for reducing variability when exposing fish to drugs is explained. Fish exposed to various patterns of ethanol exposure were found to have altered anxiety levels during withdrawal in a light/dark scototaxic assay.
Holcombe, A., Schalomon, M., Hamilton, T. J. A Novel Method of Drug Administration to Multiple Zebrafish (Danio rerio) and the Quantification of Withdrawal. J. Vis. Exp. (93), e51851, doi:10.3791/51851 (2014).