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Flat Mount Imaging of Mouse Hud og sin søknad til Analyse av hårsekken Mønster og Sensory Axon morfologi
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1Department of Molecular Biology and Genetics,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 2Department of Neuroscience,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 3Department of Ophthalmology,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
Chapters
- 00:06Title
- 02:01Dorsal Skin for Immunostaining and Melanin Imaging
- 05:40Foot Skin for the Analysis of Hair Follicle Patterning
- 06:42Foot Skin for Visualizing Sebaceous Glands
- 07:23Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization
- 08:54Imaging Hair Follicle Orientation
- 09:52Imaging and Tracing Axon Arbors
- 10:55Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts
- 12:51Conclusion
Pattedyrhud inneholder et variert utvalg av strukturer - for eksempel hårsekkene og nerveendene - som viser karakteristiske mønstre av romlige organiseringen. Analysere huden som en flat monterings utnyttet to-dimensjonal geometri i dette vev for å produsere fulltykkelse høyoppløselige bilder av hud strukturer.
