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小鼠皮肤及其毛囊图案和感觉轴突形态分析中的应用平山成像
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
DOI:

13:58 min

June 25, 2014

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Chapters

  • 00:06Title
  • 02:01Dorsal Skin for Immunostaining and Melanin Imaging
  • 05:40Foot Skin for the Analysis of Hair Follicle Patterning
  • 06:42Foot Skin for Visualizing Sebaceous Glands
  • 07:23Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization
  • 08:54Imaging Hair Follicle Orientation
  • 09:52Imaging and Tracing Axon Arbors
  • 10:55Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts
  • 12:51Conclusion

Summary

Automatic Translation

哺乳动物的皮肤中含有结构的多样化 - 如毛囊和神经末梢 - 表现出独特的空间组织模式。作为平坦安装分析皮肤借此组织的2维几何的优点,以产生皮肤结构的全厚的高清晰度图像。

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