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Tarafından Işığa Proteinler Proton Transferi ve Protein Konformasyon Dynamics Zamanda çözülmüş kızılötesi spektroskopi Fourier-dönüşümü Step-tarama
JoVE Journal
Engineering
This content is Free Access.
JoVE Journal Engineering
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Tarafından Işığa Proteinler Proton Transferi ve Protein Konformasyon Dynamics Zamanda çözülmüş kızılötesi spektroskopi Fourier-dönüşümü Step-tarama

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10:03 min

June 27, 2014

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10:03 min
June 27, 2014

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Summary

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Key steps of protein function, in particular backbone conformational changes and proton transfer reactions, often take place in the microsecond to millisecond time scale. These dynamical processes can be studied by time-resolved step-scan Fourier-transform infrared spectroscopy, in particular for proteins whose function is triggered by light.

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