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Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
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Biology
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Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

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38,687 Views

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11:49 min

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July 28, 2014

DOI:

10.3791/51516-v

DOI:
10.3791/51516-v

•

11:49 min
July 28, 2014

•

13 Views
, , , , , ,
1Department of Biology,KU Leuven

Summary

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The here described fluorescence-based calcium mobilization assay is a medium-throughput reverse pharmacology screening system for the identification of functionally activating ligand(s) of orphan G protein-coupled receptors (GPCRs).

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Caers, J., Peymen, K., Suetens, N., Temmerman, L., Janssen, T., Schoofs, L., Beets, I. Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay. J. Vis. Exp. (89), e51516, doi:10.3791/51516 (2014).

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