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High Throughput Screening Kwantitatieve Expressie en zuivering Toegepast op Recombinant Disulfide-rijke Venom eiwitten die in<em> E. coli</em
JoVE Journal
Biology
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JoVE Journal Biology
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
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High Throughput Screening Kwantitatieve Expressie en zuivering Toegepast op Recombinant Disulfide-rijke Venom eiwitten die in<em> E. coli</em

Nederlands

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23,648 Views

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12:16 min

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July 30, 2014

DOI:

10.3791/51464-v

DOI:
10.3791/51464-v

•

12:16 min
July 30, 2014

•

9 Views
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1Architecture et Fonction des Macromolécules Biologiques (AFMB),Aix-Marseille Université, 2iBiTec-S, Service d’Ingénierie Moléculaire des Protéines (SIMOPRO),Commissariat à l’énergie atomique et aux énergies alternatives (CEA) Saclay, France

Summary

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Een protocol voor de kwantitatieve, high throughput screening expressie en analytische zuivering van fusie-eiwitten uit kleinschalige Escherichia coli culturen wordt beschreven en toegepast om de expressie van disulfide-rijke dierlijke gif eiwit targets.

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Saez, N. J., Nozach, H., Blemont, M., Vincentelli, R. High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli. J. Vis. Exp. (89), e51464, doi:10.3791/51464 (2014).

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