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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

English

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18,743 Views

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09:00 min

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April 22, 2014

DOI:

10.3791/51388-v

DOI:
10.3791/51388-v

•

09:00 min
April 22, 2014

•

4 Views
, , , ,
1Institute of Bioengineering and Swiss Institute of Experimental Cancer Research (ISREC),École Polytechnique Fédérale de Lausanne, 2Department of Cell and Developmental Biology and Knight Cancer Institute,Oregon Health & Science University

Summary

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The extracellular matrix undergoes substantial remodeling during wound healing, inflammation and tumorigenesis. We present a novel intravital immunofluorescence microscopy approach to visualize the dynamics of fibrillar as well as mesh-like matrix components with high spatial and temporal resolution using epifluorescence or two-photon microscopy.

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Cite this Article

Güç, E., Fankhauser, M., Lund, A. W., Swartz, M. A., Kilarski, W. W. Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy. J. Vis. Exp. (86), e51388, doi:10.3791/51388 (2014).

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