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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Chemistry
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JoVE Journal Chemistry
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

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28,375 Views

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13:57 min

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February 18, 2014

DOI:

10.3791/51383-v

DOI:
10.3791/51383-v

•

13:57 min
February 18, 2014

•

18 Views
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1Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University

Summary

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The protocols here describe kinetic assays of protein-protein interactions with Bio-layer Interferometry. F-type ATP synthase, which is involved in cellular energy metabolism, can be inhibited by its ε subunit in bacteria. We have adapted Bio-layer Interferometry to study interactions of the catalytic complex with ε’s inhibitory C-terminal domain.

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Protein-protein InteractionsAllosteric Ligand EffectsBio-layer InterferometryATP SynthaseSubunit εBacterial F-type ATP SynthaseConformational ChangeCatalytic ComplexInhibitory InteractionKineticsDrug Discovery

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Shah, N. B., Duncan, T. M. Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects. J. Vis. Exp. (84), e51383, doi:10.3791/51383 (2014).

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