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11:18 min
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January 15, 2014
DOI:
1Department of Bioengineering,Stanford University, 2Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research,Umeå University, 3Campus de Cantoblanco,Universidad Autonoma de Madrid, 4Department of Microbiology and Immunology,Stanford University School of Medicine
Summary
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A parede celular bacteriana é composta de peptidoglycan, uma rede macromolecular de fios de açúcar cruzados por peptídeos. A Cromatografia Líquida Ultra Performance fornece alta resolução e throughput para novas descobertas da composição peptidoglycan. Apresentamos um procedimento para o isolamento das paredes celulares (sacculi) e sua posterior preparação para análise via UPLC.
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Cite this Article
Desmarais, S. M., Cava, F., de Pedro, M. A., Huang, K. C. Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography. J. Vis. Exp. (83), e51183, doi:10.3791/51183 (2014).
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