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ライブ培養神経細胞の抗体供給した後細胞表面および内在化タンパク質の異なる標識化
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Neuroscience
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JoVE Journal Neuroscience
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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ライブ培養神経細胞の抗体供給した後細胞表面および内在化タンパク質の異なる標識化

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15,226 Views

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11:56 min

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February 12, 2014

DOI:

10.3791/51139-v

DOI:
10.3791/51139-v

•

11:56 min
February 12, 2014

•

5 Views
, , , ,
1Department of Anatomy & Neuroscience,The University of Melbourne, 2Department of Neurobiology,Duke University Medical Center, 3Florey Institute of Neuroscience & Mental Health,The University of Melbourne

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Differential LabelingCell-surface ProteinsInternalized ProteinsAntibody FeedingLive Cultured NeuronsTransmembrane ReceptorProtein TraffickingEndocytosisFluorescent Secondary AntibodySubcellular Localization

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Carrodus, N. L., Teng, K. S., Munro, K. M., Kennedy, M. J., Gunnersen, J. M. Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons. J. Vis. Exp. (84), e51139, doi:10.3791/51139 (2014).

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