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임상적으로 관련동물 모델 및 3D 면역화학에 사용하기 위한 외과 표본에서 고학년 신경교종 세포 배양의 최적화
JoVE Journal
Medicine
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JoVE Journal Medicine
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry
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임상적으로 관련동물 모델 및 3D 면역화학에 사용하기 위한 외과 표본에서 고학년 신경교종 세포 배양의 최적화

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12:25 min

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January 07, 2014

DOI:

10.3791/51088-v

DOI:
10.3791/51088-v

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12:25 min
January 07, 2014

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1Department of Neurosurgery,Henry Ford Hospital

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GlioblastomaHigh-grade AstrocytomaCell CultureNeurosphere AssayCancer Stem CellsOrthotopic Xenograft3D ImmunochemistryPreclinical Models

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Hasselbach, L. A., Irtenkauf, S. M., Lemke, N. W., Nelson, K. K., Berezovsky, A. D., Carlton, E. T., Transou, A. D., Mikkelsen, T., deCarvalho, A. C. Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. J. Vis. Exp. (83), e51088, doi:10.3791/51088 (2014).

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