Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry
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January 07, 2014
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Protocol for propagation of dissociated high grade glioma surgical specimens in serum-free neurosphere medium to select for cells with cancer stem cell phenotype. For specimens that fail to grow as neurospheres, an alternative protocol is suggested. A paraffin embedding technique for maintaining the 3D neurosphere architecture for immunocytochemistry is described.
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Hasselbach, L. A., Irtenkauf, S. M., Lemke, N. W., Nelson, K. K., Berezovsky, A. D., Carlton, E. T., Transou, A. D., Mikkelsen, T., deCarvalho, A. C. Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. J. Vis. Exp. (83), e51088, doi:10.3791/51088 (2014).
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