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/
Bioengineering
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使用微流体芯片实时成像和损伤研究的响应<em>果蝇</em>幼虫
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Using Microfluidics Chips for Live Imaging and Study of Injury Responses in
Drosophila
Larvae
Please note that all translations are automatically generated.
Click here for the English version.
使用微流体芯片实时成像和损伤研究的响应<em>果蝇</em>幼虫
DOI:
10.3791/50998-v
•
11:46 min
•
February 07, 2014
•
Bibhudatta Mishra
,
Mostafa Ghannad-Rezaie
,
Jiaxing Li
,
Xin Wang
,
Yan Hao
,
Bing Ye
4
,
Nikos Chronis
5
,
Catherine A. Collins
1
Department of Molecular, Cellular and Developmental Biology
,
University of Michigan
,
2
Department of Biomedical Engineering
,
University of Michigan
,
3
Life Sciences Institute
,
University of Michigan
,
4
Department of Cell and Developmental Biology
,
University of Michigan
,
5
Department of Mechanical Engineering
,
University of Michigan
Chapters
00:05
Title
01:47
Preparing the PDMS Chip
03:47
Using the Larva Chip for Live Imaging
07:23
Inducing a Nerve Crush Injury to Larval Segmental Nerves
08:42
Immobilization Enhanced Imaging
10:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
果蝇
幼虫对于实时成像,由于其半透明的角质层和强大的遗传学一个有吸引力的模型系统。本协议描述了如何利用单层PDMS设备,被称为“幼虫芯片的细胞过程的3
龄
幼虫
果蝇
的神经元内的实时成像。
Tags
Microfluidics
Drosophila Larvae
Live Imaging
Injury Response
PDMS Microchamber
Immobilization
Nerve Cord
Segmental Nerves
Body Wall Muscles
High-resolution Imaging
Anesthetics
Physiological Processes
Longitudinal Studies
Axon Transport
Calcium Responses
Photo-convertible Proteins
Axonal Injury
Nerve Crush
Article
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