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检测现场的<em>大肠杆菌</em> O157：H7细胞的PMA-qPCR的
JoVE Journal
Biology
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JoVE Journal Biology
Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Please note that all translations are automatically generated. Click here for the English version.

检测现场的<em>大肠杆菌</em> O157：H7细胞的PMA-qPCR的

English

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16,276 Views

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08:16 min

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February 01, 2014

DOI:

10.3791/50967-v

DOI:
10.3791/50967-v

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08:16 min
February 01, 2014

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4 Views
, ,
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology,Food and Drug Administration

Summary

Automatically generated

H7定位独特的遗传标记，Z3276：一个定量PCR法检测检测大肠杆菌 O157发展。在定量PCR是结合单叠氮丙啶（PMA）处理活细胞检测。这个协议已经被修改，并适于方便和一致地处理大量样品的96孔板格式

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Live E. Coli O157:H7 DetectionORF Z3276QPCR AssayPMA-qPCRLive Cell Detection96-well Plate FormatFood SafetyEnvironmental Monitoring

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Cite this Article

Li, B., Hu, Z., Elkins, C. A. Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR. J. Vis. Exp. (84), e50967, doi:10.3791/50967 (2014).

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