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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
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February 01, 2014
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A qPCR assay was developed for detection of Escherichia coli O157:H7 targeting a unique genetic marker, Z3276. The qPCR was combined with propidium monoazide (PMA) treatment for live cell detection. This protocol has been modified and adapted to a 96-well plate format for easy and consistent handling of numerous samples
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Li, B., Hu, Z., Elkins, C. A. Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR. J. Vis. Exp. (84), e50967, doi:10.3791/50967 (2014).
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