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V3 无污渍工作流,适用于西式印迹中实用、方便和可靠的总蛋白质加载控制
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting
Please note that all translations are automatically generated.
Click here for the English version.
V3 无污渍工作流,适用于西式印迹中实用、方便和可靠的总蛋白质加载控制
DOI:
10.3791/50948-v
•
11:33 min
•
December 30, 2013
•
Anton Posch
,
Jonathan Kohn
,
Kenneth Oh
,
Matt Hammond
,
Ning Liu
1
Bio-Rad Laboratories
Chapters
00:05
Title
01:55
Gel Electrophoresis with Stain-free Gels and Ge Imaging
03:29
Protein Transfer with Trans-blot Turbo System and Antibody Incubation
06:32
Imaging and Calculating the Total Protein Normalization: Image Lab
08:53
Total Protein Normalization Results for MCM-7 in Irradiated Lymphoblastoid Cells
10:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
V3 工作流是使用无污渍凝胶的西方污点程序。无污渍技术使研究人员能够可视化蛋白质分离质量,验证转移效率,最重要的是,使用总蛋白质定量作为可靠的加载控制来验证兴趣蛋白的变化。
Tags
Western Blot
Stain-free Technology
Total Protein Loading Control
Protein Quantification
Workflow Optimization
Transfer Efficiency Verification
Housekeeping Proteins
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